A murine model of atopic dermatitis can be generated by painting the dorsal skin with hapten twice 14 days apart.

ABSTRACT: Drug development involves pharmacometric experiments in animals. Such experiments should limit animal pain and stress. Conventional murine models of atopic dermatitis (AD) used in drug development are generated by weekly painting of hapten on dorsal skin for 5 weeks. The present study aimed to develop a protocol that involves less animal distress. The experiments focused on serum total IgE levels, which are a marker of AD. The conventional protocol induced ever rising IgE levels. Experiments with extended intervals between sensitizations showed that IgE peaked ~5 days after the second sensitization, after which it returned to the control level within 12-19 days. An additional third sensitization on day 28 further increased the serum IgE level. In the 4-5 days after the second sensitization

SUBMITTER: Kitamura A 

PROVIDER: S-EPMC5902631 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature