Integrative analysis reveals functional and regulatory roles of H3K79me2 in mediating alternative splicing.

ABSTRACT:

Background

Accumulating evidence suggests alternative splicing (AS) is a co-transcriptional splicing process not only controlled by RNA-binding splicing factors, but also mediated by epigenetic regulators, such as chromatin structure, nucleosome density, and histone modification. Aberrant AS plays an important role in regulating various diseases, including cancers.

Methods

In this study, we integrated AS events derived from RNA-seq with H3K79me2 ChIP-seq data across 34 different normal and cancer cell types and found the higher enrichment of H3K79me2 in two AS types, skipping exon (SE) and alternative 3' splice site (A3SS).

Results

Interestingly, by applying self-organizing map (SOM) clustering, we unveiled two clusters mainly comprised of blood cancer cell types with

SUBMITTER: Li T 

PROVIDER: S-EPMC5902843 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature