Activity-dependent decrease in contact areas between subsurface cisterns and plasma membrane of hippocampal neurons.

ABSTRACT: Subsurface cistern (SSC) in neuronal soma and primary dendrites is a specialized compartment of endoplasmic reticulum (ER) that is in close apposition (10 nm) with the plasma membrane (PM). ER-PM contact areas are thought to be involved in intracellular calcium regulation. Here, structural changes of SSC in hippocampal neurons were examined by electron microscopy upon depolarization with high K⁺ (90 mM) or application of NMDA (50 μM) in rat dissociated cultures as well as organotypic slice cultures. The number and average length of SSC-PM contact areas in neuronal somas significantly decreased within 30 s under excitatory condition. This decrease in SSC-PM contact area progressed with time and was reversible. These results demonstrate a structural decoupling between the SSC and

SUBMITTER: Tao-Cheng JH 

PROVIDER: S-EPMC5902880 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature