The prostaglandin E₂ receptor PTGER2 and prostaglandin F_2α receptor PTGFR mediate oviductal glycoprotein 1 expression in bovine oviductal epithelial cells.

ABSTRACT: Oviductal glycoprotein 1 (OVGP1), an oviductin, is involved in the maintenance of sperm viability and motility and contributes to sperm capacitation in the oviduct. In this study, the regulatory effects exerted by prostaglandin E₂ (PGE₂) and F_2α (PGF_2α) on OVGP1 expression via their corresponding receptors in bovine oviductal epithelial cells (BOECs) were investigated. BOECs were cultured in vitro, and their expression of receptors of PGE₂ (PTGER1, PTGER2, PTGER3, and PTGER4) and PGF_2α (PTGFR) was measured using RT-qPCR. Ca²⁺ concentration was determined with a fluorescence-based method and cAMP was quantified by enzyme-linked immunosorbent assays to verify activation of PTGER2 and PTGFR by their corresponding ago

SUBMITTER: Zhang N 

PROVIDER: S-EPMC5902897 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature