MiR-148b-3p functions as a tumor suppressor in GISTs by directly targeting KIT.

ABSTRACT:

Background

Gain-of-function mutations and overexpression of KIT are characteristic features of gastrointestinal stromal tumor (GIST). Dysregulation in miRNA expression may lead to KIT overexpression and tumorigenesis.

Methods

miRNA microarray analysis and real-time PCR were used to determine the miRNA expression profiles in a cohort of 69 clinical samples including 50 CD117^IHC+/KIT^mutation GISTs and 19 CD117^IHC-/wild-type GISTs. GO enrichment and KEGG pathway analyses were performed to reveal the predicted targets of the dysregulated miRNAs. Of the dysregulated miRNAs whose expression was inversely correlated with that of KIT miRNAs were predicted by bioinformatics analysis and confirmed by luciferase reporter assay. Cell counting kit-8 (CCK-

SUBMITTER: Wang Y 

PROVIDER: S-EPMC5902930 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature