Loss of ZG16 is associated with molecular and clinicopathological phenotypes of colorectal cancer.

ABSTRACT:

Background

Zymogen granule protein 16 (ZG16) is one of the most significantly down-regulated genes in colorectal cancer (CRC) tissues. This study aimed to further evaluate its expression changes and investigate its association with molecular and clinicopathological characteristics of CRC.

Methods

We applied quantitative RT-PCR to determine expression difference between tumor and matched normal tissues from 23 CRC patients. To further validate the down-regulation in tumor tissues, we performed immunohistochemistry (IHC) analysis in 40 paraffin-embedded normal-tumor pairs and 22 colon tissues with a variety of diseases. To evaluate if the ZG16 gene changes were associated with clinicopathological characteristics, we further analyzed the gene expression and copy number changes

SUBMITTER: Meng H 

PROVIDER: S-EPMC5902988 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature