Genome-wide detection of conservative site-specific recombination in bacteria.

ABSTRACT: The ability of clonal bacterial populations to generate genomic and phenotypic heterogeneity is thought to be of great importance for many commensal and pathogenic bacteria. One common mechanism contributing to diversity formation relies on the inversion of small genomic DNA segments in a process commonly referred to as conservative site-specific recombination. This phenomenon is known to occur in several bacterial lineages, however it remains notoriously difficult to identify due to the lack of conserved features. Here, we report an easy-to-implement method based on high-throughput paired-end sequencing for genome-wide detection of conservative site-specific recombination on a single-nucleotide level. We demonstrate the effectiveness of the method by successfully detecting several novel i

SUBMITTER: Sekulovic O 

PROVIDER: S-EPMC5903667 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature