Engineering the Chloroplast Genome of Oleaginous Marine Microalga Nannochloropsis oceanica.

ABSTRACT: Plastid engineering offers an important tool to fill the gap between the technical and the enormous potential of microalgal photosynthetic cell factory. However, to date, few reports on plastid engineering in industrial microalgae have been documented. This is largely due to the small cell sizes and complex cell-wall structures which make these species intractable to current plastid transformation methods (i.e., biolistic transformation and polyethylene glycol-mediated transformation). Here, employing the industrial oleaginous microalga Nannochloropsis oceanica as a model, an electroporation-mediated chloroplast transformation approach was established. Fluorescent microscopy and laser confocal scanning microscopy confirmed the expression of the green fluorescence protein, driven by

SUBMITTER: Gan Q 

PROVIDER: S-EPMC5904192 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature