Classical NF-κB Metabolically Reprograms Sarcoma Cells Through Regulation of Hexokinase 2.

ABSTRACT:

Background

Metabolic reprogramming has emerged as a cancer hallmark, and one of the well-known cancer-associated metabolic alterations is the increase in the rate of glycolysis. Recent reports have shown that both the classical and alternative signaling pathways of nuclear factor κB (NF-κB) play important roles in controlling the metabolic profiles of normal cells and cancer cells. However, how these signaling pathways affect the metabolism of sarcomas, specifically rhabdomyosarcoma (RMS) and osteosarcoma (OS), has not been characterized.

Methods

Classical NF-κB activity was inhibited through overexpression of the IκBα super repressor of NF-κB in RMS and OS cells. Global gene expression analysis was performed using Affymetrix GeneChip Human Transcriptome Array 2.0, and data

SUBMITTER: Londhe P 

PROVIDER: S-EPMC5904193 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature