Surface pH changes suggest a role for H⁺/OH^- channels in salinity response of Chara australis.

ABSTRACT: To understand salt stress, the full impact of salinity on plant cell physiology has to be resolved. Electrical measurements suggest that salinity inhibits the proton pump and opens putative H⁺/OH^- channels all over the cell surface of salt sensitive Chara australis (Beilby and Al Khazaaly 2009; Al Khazaaly and Beilby 2012). The channels open transiently at first, causing a characteristic noise in membrane potential difference (PD), and after longer exposure remain open with a typical current-voltage (I/V) profile, both abolished by the addition of 1 mM ZnCl₂, the main known blocker of animal H⁺ channels. The cells were imaged with confocal microscopy, using fluorescein isothiocyanate (FITC) coupled to dextran 70 to illuminate the pH changes outsi

SUBMITTER: Absolonova M 

PROVIDER: S-EPMC5904247 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature