Bioorthogonal click chemistry for fluorescence imaging of choline phospholipids in plants.

ABSTRACT:

Background

Phospholipids are important structural and signaling molecules in plant membranes. Some fluorescent dyes can stain general lipids of membranes, but labeling and visualization of specific lipid classes have yet to be developed for most components of the membrane. New techniques for visualizing membrane lipids are needed to further delineate their dynamic structural and signaling roles in plant cells. In this study we examined whether propargylcholine, a bioortholog of choline, can be used to label the major membrane lipid, phosphatidylcholine, and other choline phospholipids in plants. We established that propargylcholine is readily taken up by roots, and that its incorporation is not detrimental to plant growth. After plant tissue is harvested and fixed, a click-chemistr

SUBMITTER: Paper JM 

PROVIDER: S-EPMC5905148 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature