Dynein pulling forces counteract lamin-mediated nuclear stability during nuclear envelope repair.

ABSTRACT: Recent work done exclusively in tissue culture cells revealed that the nuclear envelope (NE) ruptures and repairs in interphase. The duration of NE ruptures depends on lamins; however, the underlying mechanisms and relevance to in vivo events are not known. Here, we use the Caenorhabditis elegans zygote to analyze lamin's role in NE rupture and repair in vivo. Transient NE ruptures and subsequent NE collapse are induced by weaknesses in the nuclear lamina caused by expression of an engineered hypomorphic C. elegans lamin allele. Dynein-generated forces that position nuclei enhance the severity of transient NE ruptures and cause NE collapse. Reduction of dynein forces allows the weakened lamin network to restrict nucleo-cytoplasmic mixing and support stable NE recovery. Surpri

SUBMITTER: Penfield L 

PROVIDER: S-EPMC5905298 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature