Characterization of enterotoxin A-producing Staphylococcus aureus.

ABSTRACT:

Purpose

This study aims to characterize the wild-type staphylococcal enterotoxin A (SEA)-producing Staphylococcus aureus.

Materials and methods

We identified 29 wild-type sea-positive S. aureus isolates from dairy and meat samples, as well as from patients, measured the amount of SEA produced under favorable cultivation conditions using enzyme-linked immunosorbent assay and sea mRNA transcriptional level and investigated the phage type as well as genetic diversity by means of pulsed-field gel electrophoresis and multilocus sequence typing.

Results

Among 29 sea-positive isolates, 22 were from food sources (including one outbreak case) and seven from clinical patients. Five enterotoxin gene profiles, namely, sea (14 isolates), <

SUBMITTER: Chen D 

PROVIDER: S-EPMC5905522 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature