Using two-site binding models to analyze microscale thermophoresis data.

ABSTRACT: The emergence of microscale thermophoresis (MST) as a technique for determining the dissociation constants for bimolecular interactions has enabled these quantities to be measured in systems that were previously difficult or impracticable. However, most models for analyses of these data featured the assumption of a simple 1:1 binding interaction. The only model widely used for multiple binding sites was the Hill equation. Here, we describe two new MST analytic models that assume a 1:2 binding scheme: the first features two microscopic binding constants (K_D(1) and K_D(2)), while the other assumes symmetry in the bivalent molecule, culminating in a model with a single macroscopic dissociation constant (K_D,M) and a single factor (α) that accounts for apparent c

SUBMITTER: Tso SC 

PROVIDER: S-EPMC5906060 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature