Bioimaging with Macromolecular Probes Incorporating Multiple BODIPY Fluorophores.

ABSTRACT: Seven macromolecular constructs incorporating multiple borondipyrromethene (BODIPY) fluorophores along a common poly(methacrylate) backbone with decyl and oligo(ethylene glycol) side chains were synthesized. The hydrophilic oligo(ethylene glycol) components impose solubility in aqueous environment on the overall assembly. The hydrophobic decyl chains effectively insulate the fluorophores from each other to prevent detrimental interchromophoric interactions and preserve their photophysical properties. As a result, the brightness of these multicomponent assemblies is approximately three times greater than that of a model BODIPY monomer. Such a high brightness level is maintained even after injection of the macromolecular probes in living nematodes, allowing their visualization with a signifi

SUBMITTER: Thapaliya ER 

PROVIDER: S-EPMC5906127 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature