Structure and Properties of DNA Molecules Over The Full Range of Biologically Relevant Supercoiling States.

ABSTRACT: Topology affects physical and biological properties of DNA and impacts fundamental cellular processes, such as gene expression, genome replication, chromosome structure and segregation. In all organisms DNA topology is carefully modulated and the supercoiling degree of defined genome regions may change according to physiological and environmental conditions. Elucidation of structural properties of DNA molecules with different topology may thus help to better understand genome functions. Whereas a number of structural studies have been published on highly negatively supercoiled DNA molecules, only preliminary observations of highly positively supercoiled are available, and a description of DNA structural properties over the full range of supercoiling degree is lacking. Atomic Force Microsco

SUBMITTER: Bettotti P 

PROVIDER: S-EPMC5906655 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature