Cryopreservation of Fish Spermatogonial Cells: The Future of Natural History Collections.

ABSTRACT: As global biodiversity declines, the value of biological collections increases. Cryopreserved diploid spermatogonial cells meet two goals: to yield high-quality molecular sequence data; and to regenerate new individuals, hence potentially countering species extinction. Cryopreserved spermatogonial cells that allow for such mitigative measures are not currently in natural history museum collections because there are no standard protocols to collect them. Vertebrate specimens, especially fishes, are traditionally formalin-fixed and alcohol-preserved which makes them ideal for morphological studies and as museum vouchers, but inadequate for molecular sequence data. Molecular studies of fishes routinely use tissues preserved in ethanol; yet tissues preserved in this way may yield degraded sequ

SUBMITTER: Hagedorn MM 

PROVIDER: S-EPMC5906666 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature