Spatial Systems Lipidomics Reveals Nonalcoholic Fatty Liver Disease Heterogeneity.

ABSTRACT: Hepatocellular lipid accumulation characterizes nonalcoholic fatty liver disease (NAFLD). However, the types of lipids associated with disease progression are debated, as is the impact of their localization. Traditional lipidomics analysis using liver homogenates or plasma dilutes and averages lipid concentrations, and does not provide spatial information about lipid distribution. We aimed to characterize the distribution of specific lipid species related to NAFLD severity by performing label-free molecular analysis by mass spectrometry imaging (MSI). Fresh frozen liver biopsies from obese subjects undergoing bariatric surgery ( n = 23) with various degrees of NAFLD were cryosectioned and analyzed by matrix-assisted laser desorption/ionization (MALDI)-MSI. Molecular identification was veri

SUBMITTER: Scupakova K 

PROVIDER: S-EPMC5906754 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature