Successful application of human-based methyl capture sequencing for methylome analysis in non-human primate models.

ABSTRACT:

Background

The characterization of genomic or epigenomic variation in human and animal models could provide important insight into pathophysiological mechanisms of various diseases, and lead to new developments in disease diagnosis and clinical intervention. The African green monkey (AGM; Chlorocebus aethiops) and cynomolgus monkey (CM; Macaca fascicularis) have long been considered important animal models in biomedical research. However, non-human primate-specific methods applicable to epigenomic analyses in AGM and CM are lacking. The recent development of methyl-capture sequencing (MC-seq) has an unprecedented advantage of cost-effectiveness, and further allows for extending the methylome coverage compared to conventional sequencing approaches.

Results

Here, we used a hum

SUBMITTER: Lee JR 

PROVIDER: S-EPMC5907189 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature