Genetic characterization of a novel picorna-like virus in Culex spp. mosquitoes from Mozambique.

ABSTRACT:

Background

Mosquitoes are the potential vectors for a variety of viruses that can cause diseases in the human and animal populations. Viruses in the order Picornavirales infect a broad range of hosts, including mosquitoes. In this study, we aimed to characterize a novel picorna-like virus from the Culex spp. of mosquitoes from the Zambezi Valley of Mozambique.

Methods

The extracted RNA from mosquito pools was pre-amplified with the sequence independent single primer amplification (SISPA) method and subjected to high-throughput sequencing using the Ion Torrent platform. Reads that are classified as Iflaviridae, Picornaviridae and Dicistroviridae were assembled by CodonCode Aligner and SPAdes. Gaps between the viral contigs were sequenced by PCR. The genomic ends were analyzed

SUBMITTER: Cholleti H 

PROVIDER: S-EPMC5907373 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature