Salivary extracellular vesicle-associated miRNAs as potential biomarkers in oral squamous cell carcinoma.

ABSTRACT:

Background

Several studies in the past have investigated the expression of micro RNAs (miRNAs) in saliva as potential biomarkers. Since miRNAs associated with extracellular vesicles (EVs) are known to be protected from enzymatic degradation, we evaluated whether salivary EVs from patients with oral squamous cell carcinoma (OSCC) were enriched with specific subsets of miRNAs.

Methods

OSCC patients and controls were matched with regards to age, gender and risk factors. Total RNA was extracted from salivary EVs and the differential expression of miRNAs was evaluated by qRT-PCR array and qRT-PCR. The discrimination power of up-regulated miRNAs as biomarkers in OSCC patients versus controls was evaluated by the Receiver Operating Characteristic (ROC) curves.

Results

A prel

SUBMITTER: Gai C 

PROVIDER: S-EPMC5907383 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature