A Recurrent Silent Mutation Implicates fecA in Ethanol Tolerance by Escherichia coli.

ABSTRACT:

Background

An issue associated with efficient bioethanol production is the fact that the desired product is toxic to the biocatalyst. Among other effects, ethanol has previously been found to influence the membrane of E. coli in a dose-dependent manner and induce changes in the lipid composition of the plasma membrane. We describe here the characterization of a collection of ethanol-tolerant strains derived from the ethanologenic Escherichia coli strain FBR5.

Results

Membrane permeability assays indicate that many of the strains in the collection have alterations in membrane permeability and/or responsiveness of the membrane to environmental changes such as temperature shifts or ethanol exposure. However, analysis of the strains by gas chromatography and mass spectrometry re

SUBMITTER: Lupino KM 

PROVIDER: S-EPMC5907409 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature