Whole-genome sequencing and antigenic analysis of the first equine influenza virus identified in Turkey.

ABSTRACT:

Background

In 2013, there was an outbreak of acute respiratory disease in racehorses in Turkey. The clinical signs were consistent with equine influenza (EI).

Objective

The aim was to confirm the cause of the outbreak and characterise the causal virus.

Methods

A pan-reactive influenza type A real-time RT-PCR and a rapid antigen detection kit were used for confirmatory diagnosis of equine influenza virus (EIV). Immunological susceptibility to EIV was examined using single radial haemolysis and ELISA. Antigenic characterisation was completed by haemagglutinin inhibition using a panel of specific ferret antisera. Genetic characterisation was achieved by whole-genome sequencing using segment-specific primers with M13 tags.

Results

A H3N8 EIV of the Florida clade 2

SUBMITTER: Gahan J 

PROVIDER: S-EPMC5907808 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature