Detection of prostate cancer-specific transcripts in extracellular vesicles isolated from post-DRE urine.

ABSTRACT:

Background

The measurement of gene expression in post-digital rectal examination (DRE) urine specimens provides a non-invasive method to determine a patient's risk of prostate cancer. Many currently available assays use whole urine or cell pellets for the analysis of prostate cancer-associated genes, although the use of extracellular vesicles (EVs) has also recently been of interest. We investigated the expression of prostate-, kidney-, and bladder-specific transcripts and known prostate cancer biomarkers in urine EVs.

Methods

Cell pellets and EVs were recovered from post-DRE urine specimens, with the total RNA yield and quality determined by Bioanalyzer. The levels of prostate, kidney, and bladder-associated transcripts in EVs were assessed by TaqMan qPCR and targeted seque

SUBMITTER: Pellegrini KL 

PROVIDER: S-EPMC5907935 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature