Caveolin-1 down-regulation is required for Wnt5a-Frizzled 2 signalling in Ha-Ras^V12 -induced cell transformation.

ABSTRACT: Caveolin-1 (Cav1) is down-regulated during MK4 (MDCK cells harbouring inducible Ha-Ras^V12 gene) transformation by Ha-Ras^V12 . Cav1 overexpression abrogates the Ha-Ras^V12 -driven transformation of MK4 cells; however, the targeted down-regulation of Cav1 is not sufficient to mimic this transformation. Cav1-silenced cells, including MK4/shCav1 cells and MDCK/shCav1 cells, showed an increased cell area and discontinuous junction-related proteins staining. Cellular and mechanical transformations were completed when MDCK/shCav1 cells were treated with medium conditioned by MK4 cells treated with IPTG (MK4+I-CM) but not with medium conditioned by MK4 cells. Nanoparticle tracking analysis showed that Ha-Ras^V12 -inducing MK4 cells increased exosome-like m

SUBMITTER: Lin HK 

PROVIDER: S-EPMC5908114 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature