⁸⁹Zr-nimotuzumab for immunoPET imaging of epidermal growth factor receptor I.

ABSTRACT:

Rationale

Epidermal growth factor receptor (EGFR) upregulation is associated with enhanced proliferation and drug resistance in a number of cancers. Nimotuzumab is a humanized monoclonal antibody with high affinity for EGFR. The objective of this study was to determine if ⁸⁹Zr-DFO-nimotuzumab could be suitable for human use as a PET probe for quantifying EGFR in vivo.

Methods

To evaluate the pharmacokinetics, biodistribution, microPET imaging, radiation dosimetry, and normal tissue toxicity in tumor and non-tumor bearing mice of ⁸⁹Zr-desferoxamine-nimotuzumab (⁸⁹Zr-DFO-nimotuzumab) of a product prepared under GMP conditions. Nimotuzumab was conjugated to DFO and radiolabeled with ⁸⁹Zr. ⁸⁹Zr-DFO-nimotuzumab was cha

SUBMITTER: Chekol R 

PROVIDER: S-EPMC5908310 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature