Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance.

ABSTRACT: Candida glabrata can rapidly acquire mutations that result in drug resistance, especially to azoles and echinocandins. Identification of genetic mutations is essential, as resistance detected in vitro can often be correlated with clinical failure. We examined the feasibility of using whole genome sequencing (WGS) for genome-wide analysis of antifungal drug resistance in C. glabrata. The aim was torecognize enablers and barriers in the implementation WGS and measure its effectiveness. This paper outlines the key quality control checkpoints and essential components of WGS methodology to investigate genetic markers associated with reduced susceptibility to antifungal agents. It also estimates the accuracy of data analysis and turn-around-time of testing. Phenotypic susceptibility of 12 clinic

SUBMITTER: Biswas C 

PROVIDER: S-EPMC5908403 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature