Biological and therapeutic implications of multisector sequencing in newly diagnosed glioblastoma.

ABSTRACT:

Background

Diagnostic workflows for glioblastoma (GBM) patients increasingly include DNA sequencing-based analysis of a single tumor site following biopsy or resection. We hypothesized that sequencing of multiple sectors within a given tumor would provide a more comprehensive representation of the molecular landscape and potentially inform therapeutic strategies.

Methods

Ten newly diagnosed, isocitrate dehydrogenase 1 (IDH1) wildtype GBM tumor samples were obtained from 2 (n = 9) or 4 (n = 1) spatially distinct tumor regions. Tumor and matched blood DNA samples underwent whole-exome sequencing.

Results

Across all 10 tumors, 51% of mutations were clonal and 3% were subclonal and shared in different sectors, whereas 46% of mutations were subclonal and private. Two of th

SUBMITTER: Mahlokozera T 

PROVIDER: S-EPMC5909635 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature