Bioinformatics-based identification of potential microRNA biomarkers in frequent and non-frequent exacerbators of COPD.

ABSTRACT:

Objectives

MicroRNAs (miRNAs) play essential roles in the development of COPD. In this study, we aimed to identify and validate potential miRNA biomarkers in frequent and non-frequent exacerbators of COPD patients using bioinformatic analysis.

Materials and methods

The candidate miRNA biomarkers in COPD were screened from Gene Expression Omnibus (GEO) dataset and identified using GEO2R online tool. Then, we performed bioinformatic analyses including target prediction, gene ontology (GO), pathway enrichment analysis and construction of protein-protein interaction (PPI) network. Furthermore, the expression of the identified miRNAs in peripheral blood monocular cells (PBMCs) of COPD patients was validated using quantitative real-time polymerase chain reaction (qRT-PCR).

Resu

SUBMITTER: Liu X 

PROVIDER: S-EPMC5909781 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature