1H Magnetic Resonance Spectroscopy of live human sperm.

ABSTRACT:

Study question

Can 1H Magnetic Resonance Spectroscopy (MRS) be used to obtain information about the molecules and metabolites in live human spermatozoa?

Summary answer

Percoll-based density gradient centrifugation (DGC) followed by a further two washing steps, yielded enough sperm with minimal contamination (<0.01%) from seminal fluid to permit effective MRS which detected significant differences (P < 0.05) in the choline/glycerophosphocholine (GPC), lipid and lactate regions of the 1H MRS spectrum between sperm in the pellet and those from the 40%/80% interface.

What is known already

Current methods to examine sperm are either limited in their value (e.g. semen analysis) or are destructive (e.g. immunohistochemistry, sperm DNA testing). A few studies have previously

SUBMITTER: Reynolds S 

PROVIDER: S-EPMC5909857 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature