Treatment with AICAR inhibits blastocyst development, trophectoderm differentiation and tight junction formation and function in mice.

ABSTRACT:

Study question

What is the impact of adenosine monophosphate-activated protein kinase (AMPK) activation on blastocyst formation, gene expression, and tight junction formation and function?

Summary answer

AMPK activity must be tightly controlled for normal preimplantation development and blastocyst formation to occur.

What is known already

AMPK isoforms are detectable in oocytes, cumulus cells and preimplantation embryos. Cultured embryos are subject to many stresses that can activate AMPK.

Study design, size, duration

Two primary experiments were carried out to determine the effect of AICAR treatment on embryo development and maintenance of the blastocoel cavity. Embryos were recovered from superovulated mice. First, 2-cell embryos were treated with a concentra

SUBMITTER: Calder MD 

PROVIDER: S-EPMC5909861 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature