Human kallikrein-related peptidase 12 stimulates endothelial cell migration by remodeling the fibronectin matrix.

ABSTRACT: Kallikrein-related peptidase 12 (KLK12) is a kallikrein family peptidase involved in angiogenesis - a complex biological process in which the sprouting, migration and stabilization of endothelial cells requires extracellular matrix remodeling. To characterize the molecular mechanisms associated with KLK12's proangiogenic activity, we evaluated its ability to hydrolyze various matrix proteins. Our results show that KLK12 efficiently cleaved the human extracellular matrix proteins fibronectin and tenascin, both of which are involved in the regulation of endothelial cell adhesion and migration. For fibronectin, the major proteolytic product generated by KLK12 was a 29 kDa fragment containing the amino-terminal domain and the first five type I fibronectin-domains, which are essential for regul

SUBMITTER: Kryza T 

PROVIDER: S-EPMC5910384 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature