Observation of acetyl phosphate formation in mammalian mitochondria using real-time in-organelle NMR metabolomics.

ABSTRACT: Recent studies point out the link between altered mitochondrial metabolism and cancer, and detailed understanding of mitochondrial metabolism requires real-time detection of its metabolites. Employing heteronuclear 2D NMR spectroscopy and ¹³C₃-pyruvate, we propose in-organelle metabolomics that allows for the monitoring of mitochondrial metabolic changes in real time. The approach identified acetyl phosphate from human mitochondria, whose production has been largely neglected in eukaryotic metabolism since its first description about 70 years ago in bacteria. The kinetic profile of acetyl phosphate formation was biphasic, and its transient nature suggested its role as a metabolic intermediate. The method also allowed for the estimation of pyruvate dehydrogenase (PDH)

SUBMITTER: Xu WJ 

PROVIDER: S-EPMC5910857 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature