Parental genetic material and oxygen concentration affect hatch dynamics of mouse embryo in vitro.

ABSTRACT:

Background

Hatching is crucial for mammalian embryo implantation, since difficulties during this process can lead to implantation failure, ectopic pregnancy and consequent infertility. Despite years of intensive researches, how internal and external factors affecting embryo hatch are still largely unclear.

Methods

The effects of parental genetic material and oxygen concentration on hatch process were examined. Fertilized and parthenogenetic mouse preimplantation embryos were cultured in vitro under 5 and 20% oxygen for 120 h. Zona pellucida drilling by Peizo micromanipulation were performed to resemble the breach by sperm penetration.

Results

Firstly, parthenogenetic embryos had similarly high blastocyst developmental efficiency as fertilized embryos, but significantl

SUBMITTER: Zhan S 

PROVIDER: S-EPMC5911372 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature