High throughput measurement of metabolism in planarians reveals activation of glycolysis during regeneration.

ABSTRACT: Planarians are outstanding models for studying mechanisms of regeneration; however, there are few methods to measure changes in their metabolism. Examining metabolism in planarians is important because the regenerative process is dependent on numerous integrated metabolic pathways, which provide the energy required for tissue repair as well as the ability to synthesize the cellular building blocks needed to form new tissue. Therefore, we standardized an extracellular flux analysis method to measure mitochondrial and glycolytic activity in live planarians during normal growth as well as during regeneration. Small, uninjured planarians showed higher rates of oxygen consumption compared with large planarians, with no difference in glycolytic activity; however, glycolysis increased during plan

SUBMITTER: Osuma EA 

PROVIDER: S-EPMC5911454 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature