Investigating cross-contamination by yeast strains from dental solid waste to waste-handling workers by DNA sequencing.
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ABSTRACT: Trying to widen the discussion on the risks associated with dental waste, this study proposed to investigate and genetically compare yeast isolates recovered from dental solid waste and waste workers. Three samples were collected from workers' hands, nasal mucosa, and professional clothing (days 0, 30, and 180), and two from dental waste (days 0 and 180). Slide culture, microscopy, antifungal drug susceptibility, intersimple sequence repeat analysis, and amplification and sequencing of internal transcribed spacer regions were performed. Yeast strains were recovered from all waste workers' sites, including professional clothes, and from waste. Antifungal susceptibility testing demonstrated that some yeast recovered from employees and waste exhibited nonsusceptible profiles. The dendrogram d
SUBMITTER: Vieira CD
PROVIDER: S-EPMC5911987 | biostudies-literature | 2018 Apr
REPOSITORIES: biostudies-literature
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