The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions.

ABSTRACT: Biosensors are becoming increasingly important and implemented in various fields such as pathogen detection, molecular diagnosis, environmental monitoring, and food safety control. In this context, we used β-lactamases as efficient reporter enzymes in several protein-protein interaction studies. Furthermore, their ability to accept insertions of peptides or structured proteins/domains strongly encourages the use of these enzymes to generate chimeric proteins. In a recent study, we inserted a single-domain antibody fragment into the Bacillus licheniformis BlaP β-lactamase. These small domains, also called nanobodies, are defined as the antigen-binding domains of single chain antibodies from camelids. Like common double chain antibodies, they show high affinities and specificities for their

SUBMITTER: Vandevenne M 

PROVIDER: S-EPMC5912309 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature