Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus.

ABSTRACT: The creation of mutant lines by genome editing is accelerating genetic analysis in many organisms. CRISPR/Cas9 methods have been adapted for use in the African clawed frog, Xenopus, a longstanding model organism for biomedical research. Traditional breeding schemes for creating homozygous mutant lines with CRISPR/Cas9-targeted mutagenesis have several time-consuming and laborious steps. To facilitate the creation of mutant embryos, particularly to overcome the obstacles associated with knocking out genes that are essential for embryogenesis, a new method called leapfrogging was developed. This technique leverages the robustness of Xenopus embryos to "cut and paste" embryological methods. Leapfrogging utilizes the transfer of primordial germ cells (PGCs) from efficiently-mutagenized donor e

SUBMITTER: Blitz IL 

PROVIDER: S-EPMC5912334 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature