Oxidized phosphatidylcholines are produced in renal ischemia reperfusion injury.

ABSTRACT:

Background

The aim of this study was to determine the individual oxidized phosphatidylcholine (OxPC) molecules generated during renal ischemia/ reperfusion (I/R) injury.

Methods

Kidney ischemia was induced in male Sprague-Dawley rats by clamping the left renal pedicle for 45 min followed by reperfusion for either 6h or 24h. Kidney tissue was subjected to lipid extraction. Phospholipids and OxPC species were identified and quantitated using liquid chromatography coupled to electrospray ionization tandem mass spectrometry using internal standards.

Result

We identified fifty-five distinct OxPC in rat kidney following I/R injury. These included a variety of fragmented (aldehyde and carboxylic acid containing species) and non-fragmented products. 1-stearoyl-2-linoleoyl-pho

SUBMITTER: Solati Z 

PROVIDER: S-EPMC5912739 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature