Genes affecting progression of bacteriophage P22 infection in Salmonella identified by transposon and single gene deletion screens.

ABSTRACT: Bacteriophages rely on their hosts for replication, and many host genes critically determine either viral progeny production or host success via phage resistance. A random insertion transposon library of 240,000 mutants in Salmonella enterica serovar Typhimurium was used to monitor effects of individual bacterial gene disruptions on bacteriophage P22 lytic infection. These experiments revealed candidate host genes that alter the timing of phage P22 propagation. Using a False Discovery Rate of < 0.1, mutations in 235 host genes either blocked or delayed progression of P22 lytic infection, including many genes for which this role was previously unknown. Mutations in 77 genes reduced the survival time of host DNA after infection, including mutations in genes for enterobacterial common antigen

SUBMITTER: Bohm K 

PROVIDER: S-EPMC5912970 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature