Electron cryotomography of Mycoplasma pneumoniae mutants correlates terminal organelle architectural features and function.

ABSTRACT: The Mycoplasma pneumoniae terminal organelle functions in adherence and gliding motility and is comprised of at least eleven substructures. We used electron cryotomography to correlate impaired gliding and adherence function with changes in architecture in diverse terminal organelle mutants. All eleven substructures were accounted for in the prkC, prpC and P200 mutants, and variably so for the HMW3 mutant. Conversely, no terminal organelle substructures were evident in HMW1 and HMW2 mutants. The P41 mutant exhibits a terminal organelle detachment phenotype and lacked the bowl element normally present at the terminal organelle base. Complementation restored this substructure, establishing P41 as either a component of the bowl element or required for its assembly or stability, and that this

SUBMITTER: Krause DC 

PROVIDER: S-EPMC5912986 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature