A microfluidic device for studying chemotaxis mechanism of bacterial cancer targeting.

ABSTRACT: Bacterial cancer targeting may become an efficacious cancer therapy, but the mechanisms underlying bacterial specificity for cancer cells need to be explored prior to adopting it as a new clinical application. To characterize the mechanism of bacterial chemotactic preference towards cancer cells, we developed a microfluidic device for in vitro study. The device consists of a cell culture chamber on both sides of a central bacteria channel, with micro-channels used as barriers between them. The device, when used as model for lung cancer, was able to provide simultaneous three-dimensional co-culture of multiple cell lines in separate culture chambers, and when used as model for bacterial chemotaxis, established constant concentration gradients of biochemical compounds in a central channel by

SUBMITTER: Song J 

PROVIDER: S-EPMC5913277 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature