Development of new method to enrich human iPSC-derived renal progenitors using cell surface markers.

ABSTRACT: Cell therapy using renal progenitors differentiated from human embryonic stem cells (hESCs) or induced pluripotent stem cells (hiPSCs) has the potential to significantly reduce the number of patients receiving dialysis therapy. However, the differentiation cultures may contain undifferentiated or undesired cell types that cause unwanted side effects, such as neoplastic formation, when transplanted into a body. Moreover, the hESCs/iPSCs are often genetically modified in order to isolate the derived renal progenitors, hampering clinical applications. To establish an isolation method for renal progenitors induced from hESCs/iPSCs without genetic modifications, we screened antibodies against cell surface markers. We identified the combination of four markers, CD9^-CD140a⁺C

SUBMITTER: Hoshina A 

PROVIDER: S-EPMC5913312 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature