An evaluation of the operational model when applied to quantify functional selectivity.

ABSTRACT:

Background and purpose

Functional selectivity describes the ability of ligands to differentially regulate multiple signalling pathways when coupled to a single receptor, and the operational model is commonly used to analyse these data. Here, we assess the mathematical properties of the operational model and evaluate the outcomes of fixing parameters on model performance.

Experimental approach

The operational model was evaluated using both a mathematical identifiability analysis and simulation.

Key results

Mathematical analysis revealed that the parameters R₀ and K_E were not independently identifiable which can be solved by considering their ratio, τ. The ratio parameter, τ, was often imprecisely estimated when only functional assay data were avai

SUBMITTER: Zhu X 

PROVIDER: S-EPMC5913411 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature