Two-Stage Isothermal Enzymatic Amplification for Concurrent Multiplex Molecular Detection.

ABSTRACT:

Background

The wide array of pathogens responsible for infectious diseases makes it difficult to identify causative pathogens with single-plex tests. Although multiplex PCR detects multiple targets, it is restricted to centralized laboratories, which delays test results or makes multiplexing unavailable, depriving healthcare providers of critical, real-time information.

Methods

To address the need for point-of-care (POC) highly multiplexed tests, we propose the 2-stage, nested-like, rapid (<40 min) isothermal amplification assay, dubbed rapid amplification (RAMP). RAMP's first-stage uses outer loop-mediated isothermal amplification (LAMP) primers to amplify all targets with recombinase polymerase amplification (RPA). First-stage amplicons are aliquoted to second stage reacto

SUBMITTER: Song J 

PROVIDER: S-EPMC5913740 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature