A RecET-assisted CRISPR-Cas9 genome editing in Corynebacterium glutamicum.

ABSTRACT:

Background

Extensive modification of genome is an efficient manner to regulate the metabolic network for producing target metabolites or non-native products using Corynebacterium glutamicum as a cell factory. Genome editing approaches by means of homologous recombination and counter-selection markers are laborious and time consuming due to multiple round manipulations and low editing efficiencies. The current two-plasmid-based CRISPR-Cas9 editing methods generate false positives due to the potential instability of Cas9 on the plasmid, and require a high transformation efficiency for co-occurrence of two plasmids transformation.

Results

Here, we developed a RecET-assisted CRISPR-Cas9 genome editing method using a chromosome-borne Cas9-RecET and a single plasmid harboring sgRN

SUBMITTER: Wang B 

PROVIDER: S-EPMC5913818 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature