Enterococcus faecalis Demonstrates Pathogenicity through Increased Attachment in an <i>Ex Vivo</i> Polymicrobial Pulpal Infection.

ABSTRACT: This study investigated the host response to a polymicrobial pulpal infection consisting of Streptococcus anginosus and Enterococcus faecalis, bacteria commonly implicated in dental abscesses and endodontic failure, using a validated ex vivo rat tooth model. Tooth slices were inoculated with planktonic cultures of S. anginosus or E. faecalis alone or in coculture at S. anginosus/E. faecalis ratios of 50:50 and 90:10. Attachment was semiquantified by measuring the area covered by fluorescently labeled bacteria. Host response was established by viable histological cell counts, and inflammatory response was measured using reverse transcription-quantitative PCR (RT-qPCR) and immunohistochemistry. A significant reduction in cell viability was obs

SUBMITTER: Nishio Ayre W 

PROVIDER: S-EPMC5913860 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature