Establishment of a stable transfection system for genetic manipulation of Babesia gibsoni.

ABSTRACT:

Background

Genetic manipulation techniques, such as transfection, have been previously reported in many protozoan parasites. In Babesia, stable transfection systems have only been established for bovine Babesia parasites. We recently reported a transient transfection system and the selection of promoter candidates for Babesia gibsoni. The establishment of a stable transfection system for B. gibsoni is considered to be urgent to improve our understanding of the basic biology of canine Babesia parasites for a better control of babesiosis.

Results

GFP-expressing parasites were observed by fluorescence microscopy as early as two weeks after drug selection, and consistently expressed GFP for more than 3 months without drug pressure. Genome integration was confirmed by PCR, sequen

SUBMITTER: Liu M 

PROVIDER: S-EPMC5914073 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature