A chemiluminescent probe for cellular peroxynitrite using a self-immolative oxidative decarbonylation reaction.

ABSTRACT: Peroxynitrite (ONOO-) is a highly reactive oxygen species which has been recognized as an endogenous mediator of physiological activities like the immune response as well as a damaging agent of oxidative stress under pathological conditions. While its biological importance is becoming clearer, many of the details of its production and mechanism of action remain elusive due to the lack of available selective and sensitive detection methods. Herein, we report the development, characterization, and biological applications of a reaction-based chemiluminescent probe for ONOO- detection, termed as PNCL. PNCL reacts with ONOO-via an isatin moiety through an oxidative decarbonylation reaction to initiate light emission that can be observed instantly with high selectivity against other reactive sul

SUBMITTER: Cao J 

PROVIDER: S-EPMC5914148 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature