Insertion of inter-domain linkers improves expression and bioactivity of Zygote arrest (Zar) fusion proteins.

ABSTRACT: Developmentally important proteins that are crucial for fertilization and embryogenesis are synthesized through highly regulated translation of maternal mRNA. The Zygote arrest proteins, Zar1 and Zar2, are crucial for embryogenesis and have been implicated in binding mRNA and repressing mRNA translation. To investigate Zar1 and Zar2, the full-length proteins had been fused to glutathione-S-transferase (GST) or MS2 protein tags with minimal inter-domain linkers derived from multiple cloning sites; however, these fusion proteins expressed poorly and/or lacked robust function. Here, we tested the effect of inserting additional linkers between the fusion domains. Three linkers were tested, each 17 amino acids long with different physical and chemical properties: flexible hydrophilic, rigid ext

SUBMITTER: Cook JM 

PROVIDER: S-EPMC5914382 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature